7O9X

Crystal structure of Human Menin with fragment 16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 43 2 2
Unit cell lengths	107.063, 107.063, 107.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.944 - 2.299
R-factor	0.2006
Rwork	0.198
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ddc
RMSD bond length	0.007
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.381
High resolution limit [Å]	2.299	2.299
Rmerge	0.123
Rmeas	0.130
Rpim	0.040
Number of reflections	28344	2761
<I/σ(I)>	12.4
Completeness [%]	99.9	99.42
Redundancy	10.9
CC(1/2)	0.994	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	12% PEG 3350 14% Glycerol