7O9G
Escherichia coli Ffh in complex with ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.630, 38.200, 78.440 |
Unit cell angles | 90.00, 96.27, 90.00 |
Refinement procedure
Resolution | 43.260 - 2.800 |
R-factor | 0.1952 |
Rwork | 0.192 |
R-free | 0.25980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ng1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.635 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.260 | 2.904 |
High resolution limit [Å] | 2.800 | 2.803 |
Number of reflections | 7083 | 698 |
<I/σ(I)> | 32.05 | |
Completeness [%] | 97.4 | |
Redundancy | 3.4 | |
CC(1/2) | 0.999 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 20 % (w/v) PEG 3350 |