7O84
Structure of the PL6 family alginate lyase Pedsa0632 from Pseudopedobacter saltans in complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07227 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.182, 47.583, 116.200 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 43.970 - 2.177 |
| R-factor | 0.2312 |
| Rwork | 0.228 |
| R-free | 0.28620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | native Pedsa0632 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3 (29-NOV-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 2.310 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 46036 | 7197 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M Amm chloride, 20% (w/v) PEG 3350 |
