7O7W
Crystal structure of rsEGFP2 mutant V151L in the non-fluorescent off-state the determined by serial femtosecond crystallography at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 277.15 |
| Detector technology | CCD |
| Collection date | 2018-07-28 |
| Detector | MPCCD |
| Wavelength(s) | 1.631 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.850, 62.930, 72.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.280 - 2.100 |
| R-factor | 0.1754 |
| Rwork | 0.173 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t39 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.566 |
| Data reduction software | CrystFEL (0.7.0) |
| Data scaling software | CrystFEL (0.8.0) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.280 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 14734 | 958 |
| <I/σ(I)> | 4.9 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 293 | 100 mM HEPES pH 8.0, 2 M ammonium sulphate |
