7O7T

Structure of the PL6 family alginate lyase Patl3640 from Pseudoalteromonas atlantica T6c in complex with 4-deoxy-L-erythro-5-hexoseulose uronic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 31 2 1
Unit cell lengths	78.232, 78.232, 266.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.530 - 2.050
R-factor	0.2108
Rwork	0.209
R-free	0.24310
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	Patl3640 (PDB ID 7O77)
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	2.170
High resolution limit [Å]	2.050	2.050
Number of reflections	60589	9328
<I/σ(I)>	18
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.990	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	292	0.1 M Mg acetate, 0.1 M Na nitrate, 8% (w/v) PEG 10000, 0.15 mM CYMAL-7