7O6V
Crystal structure of the VEL1 VEL polymerising domain (R643A K645D I664D mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.066, 58.053, 119.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.040 - 2.500 |
| R-factor | 0.21492 |
| Rwork | 0.214 |
| R-free | 0.24070 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.040 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13183 | 1481 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20% PEG 3350, 2M Sodium malonate pH7 |
