7O6R
Crystal structure of bovine Hsc70(aa1-554)E213A/D214A in complex with 1H-Indazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.589, 50.466, 86.958 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 24.920 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fl9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.809 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.920 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.086 | |
| Number of reflections | 37394 | 2775 |
| <I/σ(I)> | 9.2 | 2.2 |
| Completeness [%] | 97.9 | |
| Redundancy | 3 | |
| CC(1/2) | 0.996 | 0.574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | PEG 8000, TMAO, potassium chloride, HEPES-NaOH, glycerol |
