7O6A

Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 5 minutes under anaerobic environment

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.90001
Spacegroup name	F 4 3 2
Unit cell lengths	183.675, 183.675, 183.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.040 - 1.400
R-factor	0.1331
Rwork	0.132
R-free	0.16250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r03
RMSD bond length	0.009
RMSD bond angle	1.412
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.040	2.210	1.480
High resolution limit [Å]	1.400	2.100	1.400
Rmerge	0.067	0.149	0.904
Rmeas	0.070	0.193	0.944
Rpim	0.015	0.104	0.200
Number of reflections	52581	1869	7551
<I/σ(I)>	26.3	4.9	3.7
Completeness [%]	100.0	83.7	100
Redundancy	22	2.8	22.2
CC(1/2)	1.000	0.953	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0