7O64
Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 1 minute.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.168, 184.168, 184.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.590 - 1.960 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r03 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.692 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.110 | 3.000 | 2.070 |
| High resolution limit [Å] | 1.960 | 2.850 | 1.960 |
| Rmerge | 0.166 | 0.611 | 0.672 |
| Rmeas | 0.172 | 0.635 | 0.696 |
| Rpim | 0.037 | 0.140 | 0.149 |
| Number of reflections | 17419 | 936 | 2829 |
| <I/σ(I)> | 11.3 | 2.9 | 3.4 |
| Completeness [%] | 87.7 | 100 | 100 |
| Redundancy | 21 | 20.1 | 21.5 |
| CC(1/2) | 0.997 | 0.950 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |
