7O60

Crystal structure of human myelin protein P2 at room temperature from joint X-ray and neutron refinement.

Experimental procedure

Experimental method	LAUE
Source type	NUCLEAR REACTOR
Source details	ILL BEAMLINE LADI III
Synchrotron site	ILL
Beamline	LADI III
Temperature [K]	292
Detector technology	AREA DETECTOR
Collection date	2014-09-02
Detector	LADI III
Wavelength(s)	3.0-3.9
Spacegroup name	P 41 21 2
Unit cell lengths	57.952, 57.952, 100.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
R-factor	0.2995
Rwork	0.295
R-free	0.34120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.029
RMSD bond angle	2.085
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	2.530	2.060
High resolution limit [Å]	2.000	2.400	2.000
Rmeas	0.063		0.513
Number of reflections	12464	363	891
<I/σ(I)>	25.2	2.8	3
Completeness [%]	98.9	36.9	96.1
Redundancy	9.7	2.2	3.2
CC(1/2)	0.999		0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	28% PEG 6000, 0.1 M citrate, pD 4.75
1	VAPOR DIFFUSION, HANGING DROP		281	28% PEG 6000, 0.1 M citrate, pD 4.75