7O4O

Structure of Staphylococcus aureus m1A22-tRNA methyltransferase in complex with S-adenosylhomocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	90
Detector technology	PIXEL
Collection date	2017-09-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	59.750, 61.440, 63.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.370 - 1.520
R-factor	0.1724
Rwork	0.169
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292115104
RMSD bond length	0.011
RMSD bond angle	1.704
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.400	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.034	0.480
Number of reflections	36225	3577
<I/σ(I)>	15.6	1.54
Completeness [%]	99.3
Redundancy	2	2
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% PEG 10,000