7O3N
Crystal Structure of AcrB Single Mutant - 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 152.343, 157.105, 218.859 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.561 |
| R-factor | 0.3093 |
| Rwork | 0.306 |
| R-free | 0.36640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gif |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.031 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.330 | 3.770 |
| High resolution limit [Å] | 3.560 | 3.560 |
| Rpim | 0.113 | 0.415 |
| Number of reflections | 122450 | 17781 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M MES PH 6.5, 0.2 M MGAC, 10% PEG 3350 |
