7O3K
Structure of a monomeric variant (L135E) of Sandercyanin fluorescent protein bound to biliverdin IX-alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 |
| Unit cell lengths | 39.810, 39.810, 119.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.810 - 2.750 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ez2 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHENIX (1.19_4092) |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.810 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.109 | 0.553 |
| Rmeas | 0.130 | 0.657 |
| Rpim | 0.070 | 0.350 |
| Number of reflections | 4795 | 703 |
| <I/σ(I)> | 9 | 2.4 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.993 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 26mg/mL 10% (w/v) PEG 1000, 10% (w/v) PEG 8000 |
