7O3B
Crystal structure of the TTBK2-CEP164 complex bound to a camelid nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.615, 127.741, 218.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.110 - 2.400 |
| R-factor | 0.1919 |
| Rwork | 0.189 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7o0s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.623 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.800 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.705 |
| Rpim | 0.052 | 0.436 |
| Number of reflections | 38541 | 4025 |
| <I/σ(I)> | 8.6 | 2.1 |
| Completeness [%] | 98.7 | 99.4 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.993 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 100 mM Acetate, pH 4.5, 100 mM NaCl, 30% PEG-200 |
