7O3B

Crystal structure of the TTBK2-CEP164 complex bound to a camelid nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	C 2 2 21
Unit cell lengths	70.615, 127.741, 218.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.110 - 2.400
R-factor	0.1919
Rwork	0.189
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o0s
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0009)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.800	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.091	0.705
Rpim	0.052	0.436
Number of reflections	38541	4025
<I/σ(I)>	8.6	2.1
Completeness [%]	98.7	99.4
Redundancy	3.8	3.8
CC(1/2)	0.993	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	100 mM Acetate, pH 4.5, 100 mM NaCl, 30% PEG-200