7O38

cytochrome C kustc0562 from Kuenenia stuttgartiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 64
Unit cell lengths	101.650, 101.650, 28.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.000
R-factor	0.1969
Rwork	0.191
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mxy
RMSD bond length	0.013
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.020	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.037	0.166
Rmeas	0.041
Number of reflections	3395	307
<I/σ(I)>	6.5
Completeness [%]	95.8
Redundancy	2.4
CC(1/2)	0.973	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M NaCl, 30% w/v PEG 8000, 0.1 M imidazole pH 8.0, 0.277 mM cyclohexylethanoyl-N-hydroxyethylglucamide