7O28

Crystal structure of the human METTL3-METTL14 complex bound to Compound 19 (ADO_AE_009)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	63.871, 63.871, 224.515
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.400 - 2.470
R-factor	0.2069
Rwork	0.204
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7acd
RMSD bond length	0.009
RMSD bond angle	1.049
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.910	2.610
High resolution limit [Å]	2.470	2.470
Number of reflections	37082	3115
<I/σ(I)>	16.22
Completeness [%]	99.8
Redundancy	9.55
CC(1/2)	0.999	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate