7O1S
Complex-B bound [FeFe]-hydrogenase maturase HydE fromT. Maritima (Wild-type protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-21 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.270, 79.700, 86.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.050 - 1.390 |
| R-factor | 0.1562 |
| Rwork | 0.155 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ciw |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.050 | 1.480 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 102006 | 2180 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.998 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG, LiSO4; Tris Ph 7.4 |
