7O0S
Crystal structure of the N-terminal domain of CEP164(1-109) bound to camelid nanobody 36Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-04 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 38.258, 50.558, 119.867 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 59.200 - 1.700 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7o06 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.813 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.200 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.146 | 1.658 |
| Rpim | 0.061 | 0.700 |
| Number of reflections | 25042 | 1331 |
| <I/σ(I)> | 6.1 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.995 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.18 M tri-ammonium citrate, 20 % (w/v) PEG 3350 |
