7O0M

Crystal structure of the human METTL3-METTL14 complex bound to Compound 9 (ADO_AD_023)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.070, 64.070, 225.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.610 - 2.390
R-factor	0.1991
Rwork	0.197
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7acd
RMSD bond length	0.009
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.020	2.540
High resolution limit [Å]	2.390	2.390
Number of reflections	22077	3471
<I/σ(I)>	18.45
Completeness [%]	99.9
Redundancy	9.78
CC(1/2)	0.999	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate