7NZB
Mutant V517L of the SH3 domain of JNK-interacting protein 1 (JIP1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96545 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.836, 149.720, 68.094 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 67.659 - 1.959 |
| Rwork | 0.225 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nyk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.821 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.659 | 1.993 |
| High resolution limit [Å] | 1.959 | 1.959 |
| Number of reflections | 47266 | 2676 |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 97.0 | 97.8 |
| Redundancy | 2.7 | |
| CC(1/2) | 0.988 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1-5 % PEG 400, 2-2.5 M ammonium sulfate |
