7NZ7

Crystal structure of mouse ADAT2/ADAT3 tRNA deamination complex 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 32 2 1
Unit cell lengths	105.471, 105.471, 187.076
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.940 - 2.960
R-factor	0.1967
Rwork	0.195
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dh1
RMSD bond length	0.009
RMSD bond angle	1.065
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.130
High resolution limit [Å]	2.960	2.960
Rmerge	0.092	3.820
Number of reflections	25887	4112
<I/σ(I)>	26.48	1.02
Completeness [%]	99.9	99.9
Redundancy	19.7	19.7
CC(1/2)	1.000	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7% (W/V) PEG8000, 0.1 M HEPES pH 7.0, 8% ethylene glycol