7NYO
Mutant A541L of SH3 domain of JNK-interacting Protein 1 (JIP1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.873128 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.093, 67.104, 59.142 |
| Unit cell angles | 90.00, 126.70, 90.00 |
Refinement procedure
| Resolution | 47.418 - 1.400 |
| Rwork | 0.192 |
| R-free | 0.27590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nyk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.665 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.418 | 1.510 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 41884 | 2095 |
| <I/σ(I)> | 5.5 | 1.5 |
| Completeness [%] | 91.9 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.982 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1-5 % PEG 400 and 2-2.5 M ammonium sulfate |
