7NXX

Structure of Superoxide Dismutase 1 (SOD1) in complex with nanobody 2 (Nb2).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-02-07
Detector	PSI JUNGFRAU 16M
Wavelength(s)	0.97923
Spacegroup name	P 43 21 2
Unit cell lengths	115.830, 115.830, 83.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.915 - 2.189
R-factor	0.2042
Rwork	0.202
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0a
RMSD bond length	0.007
RMSD bond angle	0.915
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.570	2.260
High resolution limit [Å]	2.189	2.190
Number of reflections	29792	2455
<I/σ(I)>	12.6
Completeness [%]	99.1
Redundancy	12.8
CC(1/2)	0.998	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		273	0.1 M Hepes,1.5 M Lithium sulphate, pH 7.5