7NX5

Crystal structure of the Epstein-Barr Virus protein ZEBRA (BZLF1, Zta) bound to a methylated DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-27
Detector	MAR CCD 165 mm
Wavelength(s)	0.8730
Spacegroup name	C 1 2 1
Unit cell lengths	207.990, 26.560, 80.850
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	16.000 - 2.500
R-factor	0.2816
Rwork	0.277
R-free	0.33870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9l
RMSD bond length	0.010
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.600
High resolution limit [Å]	2.500	2.500
Rmeas	0.082	0.712
Number of reflections	15447	1710
<I/σ(I)>	12.7	2.43
Completeness [%]	98.9	99.8
Redundancy	3.632	3.724
CC(1/2)	0.999	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	22% polyethylene glycol (PEG) 4K, 18% PEG 400, 50 mM sodium acetate pH 4 and 20% isopropanol