7NWN

A carbohydrate binding module family 9 (CBM9) from Caldicellulsiruptor kristjanssonii

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	I 4 3 2
Unit cell lengths	172.671, 172.671, 172.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.191 - 1.970
Rwork	0.180
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i8u
RMSD bond length	0.013
RMSD bond angle	1.811
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.191	2.110
High resolution limit [Å]	1.970	1.970
Number of reflections	31213	5694
<I/σ(I)>	26.81
Completeness [%]	100.0
Redundancy	79.9
CC(1/2)	1.000	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M phosphate-citrate and 40% polyethylene glycol 300