7NW1

Crystal structure of UFC1 in complex with UBA5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9720
Spacegroup name	P 21 21 21
Unit cell lengths	47.204, 67.421, 133.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.761 - 1.950
R-factor	0.2185
Rwork	0.215
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z6o
RMSD bond length	0.007
RMSD bond angle	1.555
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.710	2.050
High resolution limit [Å]	1.950	1.950
Rmeas	0.132	1.870
Number of reflections	15162	152
<I/σ(I)>	11.7
Completeness [%]	46.5	3
Redundancy	8.3	6.8
CC(1/2)	0.999	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35 mM citric acid, 65 mM bis-tris propane, 19% PEG3350, 100 mM lithium chloride