7NTO
The structure of RRM domain of human TRMT2A at 1.23 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.880, 36.300, 39.100 |
| Unit cell angles | 90.00, 119.18, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.230 |
| R-factor | 0.1292 |
| Rwork | 0.127 |
| R-free | 0.17160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ntn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.129 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.260 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 17520 | 1293 |
| <I/σ(I)> | 18.2 | 1.95 |
| Completeness [%] | 97.0 | |
| Redundancy | 6.62 | |
| CC(1/2) | 1.000 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M cesium sulfate and 2.2 M ammonium sulfate |
