7NT8

Influenza virus H3N2 nucleoprotein - R416A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	87.775, 63.376, 105.950
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	86.850 - 2.220
R-factor	0.2147
Rwork	0.212
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zdp
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18rc2_3794)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.850	2.300
High resolution limit [Å]	2.220	2.220
Number of reflections	38053	203
<I/σ(I)>	8.1
Completeness [%]	90.9	55.8
Redundancy	6.6
CC(1/2)	0.992	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% w/v PEG 8000, 20% v/v ethylene glycol, 0.02 M of each alcohol (1,6-hexanediol, 0.2 M 1-butanol, 0.2 M (RS)-1,2- propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol), 0.1 M MES/imidazole pH 6.5.