7NSK
Endoplasmic reticulum aminopeptidase 2 complexed with a hydroxamic ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.559, 134.572, 128.638 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 75.559 - 3.100 |
| R-factor | 0.1907 |
| Rwork | 0.187 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ab0 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.559 | 3.172 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 42757 | 1245 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 89.0 | |
| Redundancy | 4.25 | |
| CC(1/2) | 0.789 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 10% w/v PEG 8000, 20% ethylene glycol, 0.03M of each halide (sodium salts), 0.1M MES/imidazole pH 6.5 |
