7NPO
Branched K48-K63-Ub3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-28 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.873128 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 59.630, 77.570, 50.610 |
Unit cell angles | 90.00, 123.97, 90.00 |
Refinement procedure
Resolution | 24.730 - 2.190 |
R-factor | 0.2269 |
Rwork | 0.224 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.566 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.730 | 2.268 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 9868 | 1910 |
<I/σ(I)> | 8.8 | 2.2 |
Completeness [%] | 99.6 | |
Redundancy | 2 | 2 |
CC(1/2) | 0.997 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Ammonium acetate, 20 mM Tris pH 7.5, 50 mM NaCl, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v PEG 4000; 22 mg/ml |