7NML

Galectin-1 in complex with 4-Amino-6-chloro-1,3-benzenedisulfonamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	43.319, 58.475, 112.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.400 - 1.430
R-factor	0.1775
Rwork	0.176
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q26
RMSD bond length	0.011
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.481
High resolution limit [Å]	1.430	1.430
Number of reflections	52116	32857
<I/σ(I)>	13.17
Completeness [%]	97.0
Redundancy	6.56
CC(1/2)	0.990	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	PEG 4000