7NKT

RBD domain of SARS-CoV2 in complex with neutralizing nanobody NM1226

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9999
Spacegroup name	I 41
Unit cell lengths	128.093, 128.093, 77.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.103 - 2.300
Rwork	0.184
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1z 6xc4
RMSD bond length	0.007
RMSD bond angle	1.463
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.103	2.440
High resolution limit [Å]	2.297	2.297
Number of reflections	28115	4500
<I/σ(I)>	19.5	1.4
Completeness [%]	99.9	99.6
Redundancy	9.1
CC(1/2)	0.999	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	50mM K2HPO4, 20% (w/v) PEG 8000