7NKS

Structure of the Hantaan virus Gn glycoprotein ectodomain in complex with Fab HTN-Gn1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 2
Unit cell lengths	228.022, 158.630, 70.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.010 - 3.500
R-factor	0.2262
Rwork	0.224
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5opg 5m63
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX (1.19)
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.220	3.625
High resolution limit [Å]	3.500	3.500
Rmerge	0.145	0.671
Rmeas	0.151
Rpim	0.042	0.204
Number of reflections	33293	3252
<I/σ(I)>	12.1	1.9
Completeness [%]	99.7	98.81
Redundancy	12.9
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	296	0.1 M HEPES/MOPS pH 7.5, 0.12 M monosaccharides (glucose, mannose, galactose, fucose, xylose, NAG) and 30% ppt2 (ethylene glycol and polyethylene glycol 8000)