7NHJ

Crystal structure of the human METTL3-METTL14 complex with compound DHU_M3M_154

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.180, 64.180, 225.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.690 - 2.160
R-factor	0.2203
Rwork	0.217
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l6d
RMSD bond length	0.008
RMSD bond angle	0.935
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	2.290
High resolution limit [Å]	2.160	2.160
Number of reflections	30173	4776
<I/σ(I)>	12.43
Completeness [%]	99.9
Redundancy	8.28
CC(1/2)	0.998	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate