7NHF
Crystal structure of Arabidopsis thaliana Pdx1K166R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.980023 |
| Spacegroup name | H 3 |
| Unit cell lengths | 178.001, 178.001, 115.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.380 - 2.350 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5lnu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.516 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.380 | 38.380 | 2.420 |
| High resolution limit [Å] | 2.350 | 9.970 | 2.350 |
| Rmerge | 0.112 | 0.073 | 1.033 |
| Rmeas | 0.138 | 0.088 | 1.301 |
| Rpim | 0.079 | 0.049 | 0.779 |
| Total number of observations | 2048 | 10723 | |
| Number of reflections | 55906 | 716 | 4366 |
| <I/σ(I)> | 4.6 | 9.8 | 1.3 |
| Completeness [%] | 98.7 | 96.3 | 94.4 |
| Redundancy | 2.8 | 2.9 | 2.5 |
| CC(1/2) | 0.987 | 0.986 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 28.1% PEG 1000 (w/v) and 100 mM HEPES pH 7. |
