7NH7
OC43 coronavirus methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-01 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5419 |
Spacegroup name | P 31 |
Unit cell lengths | 61.945, 61.945, 109.717 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.350 - 2.200 |
R-factor | 0.1665 |
Rwork | 0.164 |
R-free | 0.21120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yz1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.875 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.350 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.131 | |
Number of reflections | 23828 | 2385 |
<I/σ(I)> | 11.66 | 2.48 |
Completeness [%] | 99.7 | |
Redundancy | 5.1 | |
CC(1/2) | 0.981 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | JSCG screen |