7NG2
Crystal structure of Toxoplasma CPSF4-YTH domain in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 40.180, 40.180, 202.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.530 - 1.230 |
R-factor | 0.1781 |
Rwork | 0.177 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3i |
RMSD bond length | 0.005 |
RMSD bond angle | 0.771 |
Data reduction software | PHENIX |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.530 | 1.274 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 49444 | 4552 |
<I/σ(I)> | 20.3 | 1 |
Completeness [%] | 99.3 | 93.7 |
Redundancy | 11 | 4.3 |
CC(1/2) | 1.000 | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20 % PEG 4000, 20% isopropanol and 0.1M tri-sodium citrate pH 5.6 |