7NFU

Crystal structure of C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9119
Spacegroup name	P 21 3
Unit cell lengths	146.790, 146.790, 146.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.890 - 2.500
R-factor	0.2111
Rwork	0.209
R-free	0.23990
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.548
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.890	84.890	2.600
High resolution limit [Å]	2.500	9.020	2.500
Rmerge	0.091	0.024	2.654
Rmeas	0.093	0.025	2.688
Rpim	0.015	0.004	0.425
Number of reflections	36704	866	4119
<I/σ(I)>	28.5
Completeness [%]	100.0	100	100
Redundancy	39.7	35.1	39.7
CC(1/2)	1.000	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	NULL