7NF4
Structure of A. niger Fdc T395M R435P P438W variant (AnFdcII) in complex with prFMN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-28 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.450, 97.010, 177.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.070 - 1.690 |
R-factor | 0.19206 |
Rwork | 0.191 |
R-free | 0.21956 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ev4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.663 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.070 | 1.720 |
High resolution limit [Å] | 1.690 | 1.690 |
Number of reflections | 124712 | 6130 |
<I/σ(I)> | 7.8 | 1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.4 | 5.5 |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | BIS-TRIS propane, potassium thiocyanate, PEG 3350 |