7NEU
Inhibitor Complex with Thrombin Activatable Fibrinolysis Inhibitor (TAFIa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.420, 157.350, 64.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.020 - 2.800 |
| Rwork | 0.201 |
| R-free | 0.28170 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.060 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.129 | 0.491 |
| Number of reflections | 12711 | 1240 |
| <I/σ(I)> | 14.48 | 3.32 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.010 | 0.001 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 100mM MES, 300mM Ammonium Chloride, 35% PEG 3350 |
