7NE3

Human TET2 in complex with favourable DNA substrate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-22
Detector	PSI PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	C 2 2 21
Unit cell lengths	47.994, 87.711, 260.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.860 - 2.260
R-factor	0.1775
Rwork	0.175
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm6
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.260	2.260
Rmerge	0.129	1.571
Rmeas	0.133
Rpim	0.037	0.453
Number of reflections	26450	2568
<I/σ(I)>	16.4	1.5
Completeness [%]	99.9	99.2
Redundancy	13.1	12.7
CC(1/2)	0.999	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	278	0.1M MES pH6.3, 23% PEG2000MME