7NDX
Crystal structure of the human HSP40 DNAJB1-CTDs in complex with a peptide of NudC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2020-02-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 41.300, 128.264, 135.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.530 - 2.541 |
| Rwork | 0.233 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3agy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.799 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.650 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.140 | |
| Number of reflections | 12295 | 1465 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13 | |
| CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 0.1 M of sodium acetate trihydrate and 15% (v/v) of polyethylene glycol 400 |
