7NDI

Crystal structure of ZC3H12C PIN domain with Mg2+ Ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 31 2 1
Unit cell lengths	114.701, 114.701, 257.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.767 - 2.875
R-factor	0.1928
Rwork	0.192
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v34
RMSD bond length	0.002
RMSD bond angle	0.682
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	2.978
High resolution limit [Å]	2.870	2.875
Number of reflections	45274	4314
<I/σ(I)>	13.44
Completeness [%]	99.5
Redundancy	5.77
CC(1/2)	0.997	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M LiCl, 50 mM MgSO4 and 8% PEG 8000