7NC7
Crystal structure of fructose-bisphosphate aldolases FBAC from Bacillus methanolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-31 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.970, 98.260, 139.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.420 - 2.200 |
R-factor | 0.1919 |
Rwork | 0.189 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q94 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.420 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 24421 | 2158 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.8 | |
Redundancy | 4.1 | |
CC(1/2) | 0.996 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate trihydrate pH 4.5, 2.0 M ammonium sulfate |