7NB0
Structure of the DNA-binding domain of SEPALLATA 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.404, 67.436, 122.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 2.100 |
| R-factor | 0.20683 |
| Rwork | 0.204 |
| R-free | 0.23592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kov |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 1.250 |
| Rmeas | 0.084 | 1.300 |
| Number of reflections | 16555 | 1188 |
| <I/σ(I)> | 15.2 | 1.39 |
| Completeness [%] | 99.0 | 95.6 |
| Redundancy | 6 | 4.1 |
| CC(1/2) | 0.998 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | potassium sodium tartrate tetrahydrate (0.2M), bis-tris propane (0.1M, pH 7.5) and 20% PEG 3350 |
