7NAM

LRP6_E1 in complex with Lr-EET-3.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	108.890, 46.940, 87.790
Unit cell angles	90.00, 124.34, 90.00

Refinement procedure

Resolution	42.121 - 1.600
R-factor	0.1713
Rwork	0.170
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sov
RMSD bond length	0.010
RMSD bond angle	1.154
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.121	42.121	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.050	0.022	1.045
Rmeas	0.059	0.025	1.231
Total number of observations	311416
Number of reflections	91673	1057	6859
<I/σ(I)>	10.54	44.77	1.26
Completeness [%]	96.5	97.5	98.5
Redundancy	3.397	3.814	3.559
CC(1/2)	0.999	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% w/v PEG 3350, 0.2 M Na malate, pH 7.0