7N9X

CA-targeting nanobody is a tool for studying HIV-1 capsid lattice interactions

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0711
Spacegroup name	I 2 2 2
Unit cell lengths	127.950, 137.850, 313.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.581 - 3.511
Rwork	0.245
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5O2U & 3H47
RMSD bond length	0.008
RMSD bond angle	1.602
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.500	9.480	3.500
Rmerge	0.182	0.045	1.179
Rmeas	0.216	0.053	1.405
Rpim	0.114	0.027	0.748
Total number of observations	109451
Number of reflections	33153	1735	1676
<I/σ(I)>	5
Completeness [%]	95.9	93.2	98.7
Redundancy	3.3	3.3	3.3
CC(1/2)		0.996	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	100 mM Bis-Tris propane, pH 6.8, 15% PEG3350, 200 mM sodium