7N9D
I74A mutant of the isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-24 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54178 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 172.293, 172.293, 80.958 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.060 - 2.100 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lnv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.645 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.097 | 0.745 |
Number of reflections | 41416 | 1983 |
<I/σ(I)> | 12.32 | 1.189 |
Completeness [%] | 99.4 | |
Redundancy | 10.8 | |
CC(1/2) | 0.992 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 273 | 3.5 M Sodium Formate pH 7.0 |