7N6P

Crystal structure of the anti-EBOV and SUDV monoclonal antibody 1C3 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	73.885, 83.737, 133.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.674 - 2.150
R-factor	0.1776
Rwork	0.175
R-free	0.23090
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.15rc1_3420))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.740	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	45507	3452
<I/σ(I)>	8
Completeness [%]	99.2
Redundancy	8.3
CC(1/2)	0.989	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	100 mM CHES/Sodium hydroxide pH 9.5, 40% PEG 600 at 12 mg/ml