7N5W

ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing GGACCC (Oligo 23)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	74.157, 103.429, 83.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.080 - 2.240
R-factor	0.1916
Rwork	0.190
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n5u
RMSD bond length	0.006
RMSD bond angle	0.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.080	2.330
High resolution limit [Å]	2.240	2.240
Rmerge	0.124	0.904
Rpim	0.035	0.470
Number of reflections	25580	1003
<I/σ(I)>	18.3	1.2
Completeness [%]	89.5	43.6
Redundancy	11.4	2.5
CC(1/2)		0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	0.1 M sodium malonate, pH 5.0, 12% PEG3350